產(chǎn)品描述 High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. HMG-1 belongs to the high mobility group and contains a HMG-box domain. Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has been shown to play an important role in helping the RAG endonuclease form a paired complex during V(D)J recombination.

產(chǎn)品名稱 Anti-HMGB1 Recombinant Rabbit Monoclonal Antibody [SA39-03]

分子量 Predicted band size: 25 kDa

種屬反應(yīng)性 Human,Mouse,Rat

驗(yàn)證應(yīng)用 WB,IF-Cell,IF-Tissue,IHC-P,FC

抗體類型 重組兔單抗

免疫原 Synthetic peptide within Human HMGB1 aa 151-200 / 215.

偶聯(lián) Non-conjugated

性能

形態(tài) Liquid

濃度 1 mg/mL.

存放說(shuō)明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存儲(chǔ)緩沖液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亞型 IgG

純化方式 Protein A affinity purified.

亞細(xì)胞定位 Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome